Влияние предварительного замораживания и низкотемпературного хранения на взаимодействие ацетилхолинэстеразы с озоном
Vernadsky National Library of Ukraine
Переглянути архів Інформація| Поле | Співвідношення | |
| Title |
Влияние предварительного замораживания и низкотемпературного хранения на взаимодействие ацетилхолинэстеразы с озоном
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| Creator |
Грищенко, В.И.
Дюбко, Т.С. Зинченко, В.Д. |
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| Subject |
Біологія
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| Description |
Ozone (O3) effect on the conformation state and the enzyme activity of acetylcholinesterase (CE) in solutions subjected to freezing by two regimes: “slow” down to −18 ◦C (I regime) and “rapid” down to −196 ◦C (II regime) with the following storage for 1 week under corresponding temperatures has been studied by using the methods of optical pectroscopy. Freezing rate and storage temperature were established to affect the CE conformation state determining the protein sensitivity to the following ozone treatment. The enzyme samples treated according to regime I are characterized by the highest sensitivity to increasing ozone doses. A feasible relation of sensitivity to the ozone effect of CE samples, treated according to regimes I and II, with differences in the structure of molecular aggregates formed at the “rapid” and “slow” freezing of the enzyme solution is discussed. |
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| Date |
2009-07-10T12:15:29Z
2009-07-10T12:15:29Z 2007 |
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| Type |
Article
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| Identifier |
Влияние предварительного замораживания и низкотемпературного хранения на взаимодействие ацетилхолинэстеразы с озоном / В.И. Грищенко, Т.С. Прізвище, В.Д. Зинченко // Доп. НАН України. — 2007. — № 12. — С. 150-155. — Бібліогр.: 15 назв. — рос.
1025-6415 http://dspace.nbuv.gov.ua/handle/123456789/3828 535.342.372:577.322.4 |
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| Language |
ru
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| Publisher |
Видавничий дім "Академперіодика" НАН України
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