L-ARGININE, BUT NOT L-NAME PROTECTS AGAINST LIVER INJURY INDUCED BY EXPERIMENTAL ISCHEMIA-REPERFUSION
Наукові журнали Тернопільського державного медичного університету імені І.Я.Горбачевського
Переглянути архів Інформація| Поле | Співвідношення | |
| Title |
L-ARGININE, BUT NOT L-NAME PROTECTS AGAINST LIVER INJURY INDUCED BY EXPERIMENTAL ISCHEMIA-REPERFUSION
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| Creator |
Oleshchuk, O. M.; Horbachevsky Ternopil State Medical University
Posokhova, K. A. Mudra, A. Ye. |
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| Description |
Background. Hepatic ischemia-reperfusion (I/R) injury occurs upon restoration of hepatic blood flow after a period of ischemia.Objective. The study establishes that stimulation or blockade of nitric oxide synthesis has a protective effect during ischemia-reperfusion.Methods. Male albino rats which were divided into four equal groups: sham-operated control, ischemia and reperfusion group (0.9 % saline i.p.) for 3 days, group pre-treated with L-arginine (25 mg/kg i.p.), group pre-treated with L-NAME (10 mg/kg i.p.) for 3 days before ischemia-reperfusion maneuver. Complete ischemia of the median and left hepatic lobes was induced by clamping the left branches of the portal vein and the hepatic artery for 45 min. Rats were sacrificed after 3-h reperfusion. Nitric oxide synthase 3 (endothelial) and nitric oxide synthase 2 (inducible) expression, nitric oxide stabile metabolites (NO2, NO3) content, AST and ALT activities were determined. Histological examination of liver tissue was performed.Conclusions. Relative NO deficiency, due to eNOS inhibition, is central in the pathogenesis of hepatic ischemia reperfusion injury. Replacing NO content with either precursors or via donor drugs represents novel methods in ameliorating ischemia-reperfusion injury.KEY WORDS: hepatic ischemia-reperfusion, nitric oxide, NOS isoforms, L-arginine, L-NAME
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| Publisher |
Ternopil State Medical University
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| Contributor |
—
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| Date |
2014-12-25
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| Type |
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion — |
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| Format |
application/pdf
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| Identifier |
http://ojs.tdmu.edu.ua/index.php/ijmr/article/view/2820
10.11603/ijmmr.2413-6077.2015.1.2820 |
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| Source |
International Journal of Medicine and Medical Research; Том 1, № 1 (2015): Issue No 1
International Jornal of Medicine and Medical Research; Том 1, № 1 (2015): Issue No 1 International Journal of Medicine and Medical Research; Том 1, № 1 (2015): Issue No 1 2413-6077 10.11603/ijmmr.2413-6077.2015.1 |
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| Language |
eng
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| Relation |
http://ojs.tdmu.edu.ua/index.php/ijmr/article/view/2820/3143
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| Rights |
##submission.copyrightStatement##
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