ENZYME MARKERS ACTIVITY AND BILE FORMATION FUNCTION OF LIVER IN CASES OF TUBERCULOSTATICS AND HEXAVALENT CHROMIUM COMPOUNDS AFFECTION IN RATS
Наукові журнали Тернопільського державного медичного університету імені І.Я.Горбачевського
Переглянути архів Інформація| Поле | Співвідношення | |
| Title |
ENZYME MARKERS ACTIVITY AND BILE FORMATION FUNCTION OF LIVER IN CASES OF TUBERCULOSTATICS AND HEXAVALENT CHROMIUM COMPOUNDS AFFECTION IN RATS
ENZYME MARKERS ACTIVITY AND BILE FORMATION FUNCTION OF LIVER IN CASES OF TUBERCULOSTATICS AND HEXAVALENT CHROMIUM COMPOUNDS AFFECTION IN RATS |
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| Creator |
Burmas, N. I.
Fira, L. S. Lyhackyy, P. H. |
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| Description |
Background. Currently, the growing incidence of toxic lesions of the liver is associated withindustrial chemicalization and uncontrolled use of hepatotoxic drugs in everyday life. There are aboutone thousand drugs with high or low hepatotoxicity, such as anti-TB drugs.Objective. In this research we studied the intracellular enzymes activity and bile formation functionof the liver in rats of different ages in cases of tuberculostatic (isoniazid and rifampicin) affection andchromium (potassium dichromate) intoxication.Methods. The experimental affection of rats of different ages was performed by combined injectionof hexavalent chromium compounds (a solution of potassium dichromate, 3 mg/kg), isoniazid(0.05 g/kg) and rifampicin (0.25 g/kg). On the 7th and 14th days the rats were injected with enterosorbentSorbex (150 mg/kg). Enzyme markers activity of the liver was evaluated due to alanine and aspartateaminotransferases (ALT and AST) and alkaline phosphatase (ALP) rates. Bile formation function of theliver was evaluated by total bilirubin and bile acids content in blood.Results. The disorders in hepatocytes plasma membranes permeability were defined by theincreased rates of ALT, AST and alkaline phosphatase in blood serum which were decreased in theliver. It was determined that total bilirubin and bile acids content in blood serum of the affectedanimals increased. It influenced hepatocytes excretion in bile capillaries and caused cholestasis andrevenues decrease in bile.Conclusions. The most significant metabolic disorders in cases of chrome-isoniazid-rifampicinaffection were defined in immature and senior animals in comparison with mature animals.KEY WORDS: isoniazid, rifampicin, hexavalent chromium compounds, liver enzymes,bile formation.
Background. Currently, the growing incidence of toxic lesions of the liver is associated withindustrial chemicalization and uncontrolled use of hepatotoxic drugs in everyday life. There are aboutone thousand drugs with high or low hepatotoxicity, such as anti-TB drugs.Objective. In this research we studied the intracellular enzymes activity and bile formation functionof the liver in rats of different ages in cases of tuberculostatic (isoniazid and rifampicin) affection andchromium (potassium dichromate) intoxication.Methods. The experimental affection of rats of different ages was performed by combined injectionof hexavalent chromium compounds (a solution of potassium dichromate, 3 mg/kg), isoniazid(0.05 g/kg) and rifampicin (0.25 g/kg). On the 7th and 14th days the rats were injected with enterosorbentSorbex (150 mg/kg). Enzyme markers activity of the liver was evaluated due to alanine and aspartateaminotransferases (ALT and AST) and alkaline phosphatase (ALP) rates. Bile formation function of theliver was evaluated by total bilirubin and bile acids content in blood.Results. The disorders in hepatocytes plasma membranes permeability were defined by theincreased rates of ALT, AST and alkaline phosphatase in blood serum which were decreased in theliver. It was determined that total bilirubin and bile acids content in blood serum of the affectedanimals increased. It influenced hepatocytes excretion in bile capillaries and caused cholestasis andrevenues decrease in bile.Conclusions. The most significant metabolic disorders in cases of chrome-isoniazid-rifampicinaffection were defined in immature and senior animals in comparison with mature animals.KEY WORDS: isoniazid, rifampicin, hexavalent chromium compounds, liver enzymes,bile formation. |
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| Publisher |
Ternopil State Medical University
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| Contributor |
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| Date |
2016-05-12
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| Type |
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion — |
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| Format |
application/pdf
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| Identifier |
http://ojs.tdmu.edu.ua/index.php/ijmr/article/view/6379
10.11603/ijmmr.2413-6077.2016.1.6379 |
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| Source |
International Journal of Medicine and Medical Research; № 1 (2016)
International Jornal of Medicine and Medical Research; № 1 (2016) International Journal of Medicine and Medical Research; № 1 (2016) 2414-9985 2413-6077 10.11603/ijmmr.2413-6077.2015.2 |
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| Language |
ukr
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| Relation |
http://ojs.tdmu.edu.ua/index.php/ijmr/article/view/6379/5839
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| Rights |
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