DETECTION OF OXIDATIVE STRESS, APOPTOSIS AND MOLECULAR LESIONS IN HUMAN OVARIAN CANCER CELLS
Наукові журнали Тернопільського державного медичного університету імені І.Я.Горбачевського
Переглянути архів Інформація| Поле | Співвідношення | |
| Title |
DETECTION OF OXIDATIVE STRESS, APOPTOSIS AND MOLECULAR LESIONS IN HUMAN OVARIAN CANCER CELLS
DETECTION OF OXIDATIVE STRESS, APOPTOSIS AND MOLECULAR LESIONS IN HUMAN OVARIAN CANCER CELLS |
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| Creator |
Falfushynska, H. I.
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| Description |
Background. Ovarian cancer has the highest mortality rate of gynaecological cancers. This is partly due tothe lack of effective screening markers. Indices of oxidative stress are well-recognized prognostic criteria fortumorous transformation of tissue, but their value depends on the type of tumor and the stage of its development.Objective. The aim of this study is to clarify the relationship between antioxidant/pro-oxidant ratio and thesigns of molecular lesions and apoptosis rate in blood of ovarian cancer patients and non-cancer ones.Results. The ovarian cancer group is marked by antioxidant/prooxidant balance shifting to oxidative damagein blood as the consequence of overexpression of oxyradicals (by 300%). Higher level of glutathione (by 366%),lower level of metallothioneins (by 65%) as well as higher level of lipid peroxidation (by 174%) and protein carbonyls(by 186%) in blood of ovarian cancer patients compared to the normal ovarian group have been observed. Thesigns of cytotoxicity are determined in blood of ovarian cancer patients: an increased (compared to control) levelof DNA fragmentation (by 160%), choline esterase (up to twice), higher rate of both caspase dependent andcaspase independent lysosomal mediated apoptosis.Conclusions. Cathepsin D activity both total and free, choline esterase activity, TBA-reactive substance andprotein carbonyls level in blood could be used as the predictive markers of worse prognosis and the signs ofhuman ovarian cancer.KEY WORDS: ovarian cancer, oxidative stress, apoptosis, caspase-3, cathepsin D, choline esterase,metallothionein.
Background. Ovarian cancer has the highest mortality rate of gynaecological cancers. This is partly due tothe lack of effective screening markers. Indices of oxidative stress are well-recognized prognostic criteria fortumorous transformation of tissue, but their value depends on the type of tumor and the stage of its development.Objective. The aim of this study is to clarify the relationship between antioxidant/pro-oxidant ratio and thesigns of molecular lesions and apoptosis rate in blood of ovarian cancer patients and non-cancer ones.Results. The ovarian cancer group is marked by antioxidant/prooxidant balance shifting to oxidative damagein blood as the consequence of overexpression of oxyradicals (by 300%). Higher level of glutathione (by 366%),lower level of metallothioneins (by 65%) as well as higher level of lipid peroxidation (by 174%) and protein carbonyls(by 186%) in blood of ovarian cancer patients compared to the normal ovarian group have been observed. Thesigns of cytotoxicity are determined in blood of ovarian cancer patients: an increased (compared to control) levelof DNA fragmentation (by 160%), choline esterase (up to twice), higher rate of both caspase dependent andcaspase independent lysosomal mediated apoptosis.Conclusions. Cathepsin D activity both total and free, choline esterase activity, TBA-reactive substance andprotein carbonyls level in blood could be used as the predictive markers of worse prognosis and the signs ofhuman ovarian cancer.KEY WORDS: ovarian cancer, oxidative stress, apoptosis, caspase-3, cathepsin D, choline esterase,metallothionein. |
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| Publisher |
Ternopil State Medical University
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| Contributor |
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| Date |
2016-05-12
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| Type |
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion — |
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| Format |
application/pdf
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| Identifier |
http://ojs.tdmu.edu.ua/index.php/ijmr/article/view/6373
10.11603/ijmmr.2413-6077.2016.1.6373 |
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| Source |
International Journal of Medicine and Medical Research; № 1 (2016)
International Jornal of Medicine and Medical Research; № 1 (2016) International Journal of Medicine and Medical Research; № 1 (2016) 2414-9985 2413-6077 10.11603/ijmmr.2413-6077.2015.2 |
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ukr
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| Relation |
http://ojs.tdmu.edu.ua/index.php/ijmr/article/view/6373/5835
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| Rights |
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