LEVELS OF NITRIC OXIDE METABOLITES IN RATS WITH HEPATOPULMONARY SYNDROME
Наукові журнали Тернопільського державного медичного університету імені І.Я.Горбачевського
Переглянути архів Інформація| Поле | Співвідношення | |
| Title |
LEVELS OF NITRIC OXIDE METABOLITES IN RATS WITH HEPATOPULMONARY SYNDROME
LEVELS OF NITRIC OXIDE METABOLITES IN RATS WITH HEPATOPULMONARY SYNDROME |
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| Creator |
Krynytska, I. Ya.
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| Description |
Background. System of nitric oxide (NO), which consists of NO, and its metabolites, is very important forvarious biological processes. NO is signalling molecules and mediators of intracellular and intercellular interactionthat causes relaxation of smooth muscles of blood vessel walls, inhibits platelet aggregation and their adherence,is involved in the transmission of nerve impulses, cell proliferation.Objective. The aim of our research was to study the content of nitric oxide metabolites in blood serum andbronchoalveolar lavage, to substantiate their role in pathogenesis of hepatopulmonary syndrome inexperiment.Methods. The experiments were performed on 56 outbread male rats, 180-220 g in weight. The first experimentalmodel of hepatopulmonary syndrome (HPS) was made by imposition of double ligature on commonbile duct and its further dissection with a scalpel. The second experimental HPS model was made by 8-week intragastricadministration of oil solution CCl4 (400 g per 1 L), 0.5 ml per 100 g of body weight on the first day ofthe experiment, 0.3 ml per 100 g on the third day of the experiment and then every third day until the end of theexperiment 0.3 ml per 100 g. A mixture of corn flour, lard and cholesterol and alcohol solution was added to thestandard diet of the rats.Results. The total content of nitric oxide metabolites in blood serum of the rats of the experimental groupNo.1 (on the 31st day after the common bile duct ligation) was significantly increased in 3.9 times (p1<0,001) ifcompared with the control group №1. In the rats of the 2nd experimental group (with carbon tetrachloride inducedcirrhosis) the total content of nitric oxide metabolites in blood serum also significantly increased in 3.1 times(p1<0,001). Comparison of nitric oxide metabolites content in blood serum and bronchoalveolar lavage, whichdirectly indicated about the processes in lung tissue, was great importance.Conclusions. So, in rats with experimental hepatopulmonary syndrome activation of nitroxydergic processby significant increase in nitric oxide metabolites in blood serum and bronchoalveolar lavage took place.KEYWORDS: hepatopulmonary syndrome, nitric oxide metabolites.
Background. System of nitric oxide (NO), which consists of NO, and its metabolites, is very important forvarious biological processes. NO is signalling molecules and mediators of intracellular and intercellular interactionthat causes relaxation of smooth muscles of blood vessel walls, inhibits platelet aggregation and their adherence,is involved in the transmission of nerve impulses, cell proliferation.Objective. The aim of our research was to study the content of nitric oxide metabolites in blood serum andbronchoalveolar lavage, to substantiate their role in pathogenesis of hepatopulmonary syndrome inexperiment.Methods. The experiments were performed on 56 outbread male rats, 180-220 g in weight. The first experimentalmodel of hepatopulmonary syndrome (HPS) was made by imposition of double ligature on commonbile duct and its further dissection with a scalpel. The second experimental HPS model was made by 8-week intragastricadministration of oil solution CCl4 (400 g per 1 L), 0.5 ml per 100 g of body weight on the first day ofthe experiment, 0.3 ml per 100 g on the third day of the experiment and then every third day until the end of theexperiment 0.3 ml per 100 g. A mixture of corn flour, lard and cholesterol and alcohol solution was added to thestandard diet of the rats.Results. The total content of nitric oxide metabolites in blood serum of the rats of the experimental groupNo.1 (on the 31st day after the common bile duct ligation) was significantly increased in 3.9 times (p1<0,001) ifcompared with the control group №1. In the rats of the 2nd experimental group (with carbon tetrachloride inducedcirrhosis) the total content of nitric oxide metabolites in blood serum also significantly increased in 3.1 times(p1<0,001). Comparison of nitric oxide metabolites content in blood serum and bronchoalveolar lavage, whichdirectly indicated about the processes in lung tissue, was great importance.Conclusions. So, in rats with experimental hepatopulmonary syndrome activation of nitroxydergic processby significant increase in nitric oxide metabolites in blood serum and bronchoalveolar lavage took place.KEYWORDS: hepatopulmonary syndrome, nitric oxide metabolites. |
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| Publisher |
Ternopil State Medical University
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| Contributor |
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| Date |
2016-05-12
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| Type |
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion — |
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| Format |
application/pdf
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| Identifier |
http://ojs.tdmu.edu.ua/index.php/ijmr/article/view/6380
10.11603/ijmmr.2413-6077.2016.1.6380 |
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| Source |
International Journal of Medicine and Medical Research; № 1 (2016)
International Jornal of Medicine and Medical Research; № 1 (2016) International Journal of Medicine and Medical Research; № 1 (2016) 2414-9985 2413-6077 10.11603/ijmmr.2413-6077.2015.2 |
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| Language |
ukr
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| Relation |
http://ojs.tdmu.edu.ua/index.php/ijmr/article/view/6380/5841
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| Rights |
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