THE DEVELOPMENT OF CONDITIONS OF ISOLATION AND SPECTROPHOTOMETRY DETERMINATION OF METFORMIN IN BIOLOGICAL OBJECTS
Наукові журнали Тернопільського державного медичного університету імені І.Я.Горбачевського
Переглянути архів Інформація| Поле | Співвідношення | |
| Title |
THE DEVELOPMENT OF CONDITIONS OF ISOLATION AND SPECTROPHOTOMETRY DETERMINATION OF METFORMIN IN BIOLOGICAL OBJECTS
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| Creator |
Moskalenko, V. Yu.; Тернопільський державний медичний університет, Тернопіль
Merzlikin, S. I. |
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| Description |
THE DEVELOPMENT OF ISOLATION CONDITIONS AND SPECTROPHOTOMETRIC DETERMINATION OF METFORMIN IN BIOLOGICAL OBJECTS© V. Yu. Moskalenko, S. I. MerzlikinThe National University of Pharmacy, KharkivSummary. Metformin (Glucophage, Siofor) is basic preparation of modern pharmacotherapeutic scheme for treating diabetes mellitus type 2. However long-term applications, side-effects, availability, constant increasing quantity of patients with diabetes were factors, making toxicological danger of uncontrollable drug application. The main condition of conduction directed chemical-toxicological analysis of substance, that caused poisoning, was the application of special methods of its isolation from biological objects and of modern instrumental methods of analysis for its identification and quantitative determination in obtained extracts.Objective. The development of conditions of metformin isolation from liver tissues in the directed chemical-toxicological analysis and its identification in obtained extracts by spectrophotometric method.Research methods. As research object substance of metformin hydrochloride has been applicated (monography EP 01/2005:0931, producer Harman Finochem Ltd.). Identification and quantitative determination were conducted using the modified spectrophotometer SF-46 LOMO. As model biological object sample grounded pork liver, saturated with aqueous solution of preparation, has been used.Results and discussion. The individual method of isolation metformin from liver tissues has been developed. Based on sufficiently high hydrophilic properties of metformin for its allotment from biological objects water has been proposed, and for its extraction from the aqueous phase into organic one mixture i-penthanol-buthanol (1:1) at pH 13 has been proposed. It has also been proposed conditions of purification of primary aqueous extracts of co-extractive acid substances with mixture hexane-toluene (1:1), and for purification of proteins has been proposed 25 % solution of sodium chloride. This method allows allocating toxicant from biological objects up to 82 %. Developing conditions of identification and quantitative determination of metformin in extracts from biological objects with spectrophotometric method the UV-specter of toxicant solution in methanol used, measured at the wavelength 236±2 nm. Content of metformin in extracts from biological objects has been determined by building the calibration graph. The relative standard deviation at quantitative determination of toxicant was 4,08 %, and the relative uncertainty of the average value was ± 11,36 %.Conclusions. Method of metformin isolation of liver tissues, applicable for goals of directed chemical-toxicological analysis, which allows allocating up to 82 % of preparation, has been developed. For its identification and quantitative determination in extracts from biological objects spectrophotometric method has been proposed.References Sokolova L. K. Metformin: boleye 50 let dostizheniy i uspekhov / L. K. Sokolova // Endokrinologiya. – 2013. – T. 18, № 1. – S. 59-63.Merzlikin S. I. Informatsiynyy ohlyad shchodo obgruntuvannya khimiko-toksykolohichnoho doslidzhennya na metformin / S. I. Merzlikin, V. YU. Moska-lenko // Farmakolohiya ta likarsʹka toksykolohiya. – 2010. – № 1-2 (14-15). – S. 3-10.Mansurova R. G. Izolirovaniye metformina iz biologicheskogo materiala i yego identifikatsiya / R. G. Mansurova, N. V. Kubasova, Z. A. Gazizova // Su-debno-meditsinskiy zhurnal. – 2010. – № 1. – S. 5.Bolotov V. V. Primeneniye UF- i IK-spektroskopii, immunokhimicheskikh metodov analiza pri khimiko-toksikologicheskikh issledovaniyakh: metod. raz-rabotki / V. V. Bolotov, E. I. Stadnichenko, V.S. Bondar– Khar'kov; Ukr-FA, 1992. – 24 s.Vergeychik T. KH. Opredeleniye karbamazepina v trupnom materiale s is-pol'zovaniyem proizvodnoy spektrofotometrii / T. KH. Vergeychik, S. V. Shabalin // Sudebno-meditsinskaya ekspertiza. – 1993. – T. 36, № 1. – S. 32-34.Vergeychik T. KH. Osobennosti spektrofotometricheskogo opredeleniya le-karstvennykh preparatov pri khimiko-toksikologicheskikh i sudebno-khimicheskikh issledovaniyakh / T. KH. Vergeychik, V. A. Linnikova, G. B. Gus'-kova // Izvestiya vuzov. Severo-Kavkazskiy region. Tekhnicheskiye nauki. – 1993. – № 1-2. – S. 38-44.European Pharmacopoeia. – 5th ed. – Strasbourg: Council of Europe, 2008. – 3086 p.Moskalenko V. YU. Izolyuvannya metforminu z biolohichnykh obʺyektiv / V. YU. Moskalenko, S. I. Merzlikin // Ukrayinsʹkyy biofarmatsevtychnyy zhurnal. – 2013. – № 6 (29). – S. 70-74.Moskalenko V. YU. Doslidzhennya khromatohrafichnoyi povedinky metforminu v umovakh zahalʹnoho TSHKH-skryninhu likarsʹkykh rechovyn / V. YU. Moskalen-ko, S. I. Merzlikin // Farmatsevtychnyy chasopys. – 2013. – № 4 (29). – S. 58-62.Clarke’s Analysis of Drugs and Poisons: 3-d edition. –London: Pharmaceutical Press, electronic version, 2005.British Pharmacopoeia, 2009 / Her Majesty's Stationary Office, London. – 2009. – V. 1 & 2. – P. 3813-3816.
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Тернопільський державний медичний університет ім. І.Я. Горбачевського
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—
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| Date |
2016-07-08
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| Type |
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion — |
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application/pdf
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| Identifier |
http://ojs.tdmu.edu.ua/index.php/pharm-chas/article/view/6645
10.11603/2312-0967.2016.2.6645 |
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| Source |
Pharmaceutical Review; № 2 (2016)
Фармацевтичний часопис; № 2 (2016) 2414-9926 2312-0967 10.11603/2312-0967.2016.2 |
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ukr
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| Relation |
http://ojs.tdmu.edu.ua/index.php/pharm-chas/article/view/6645/6059
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