Influence of freezing down to 77.15 K on structure and antioxidant power of some proteins
Vernadsky National Library of Ukraine
Переглянути архів Інформація| Поле | Співвідношення | |
| Title |
Influence of freezing down to 77.15 K on structure and antioxidant power of some proteins
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| Creator |
Rozanova, S.L.
Narozhnyi, S.V. Nardid, O.A. |
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| Subject |
Низкоразмерные и неупорядоченные системы
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| Description |
The purpose of the present work was to investigate influence of different freeze-thawing protocols on structure and antioxidant properties of isolated proteins. In our experiments we have studied human serum albumin, human hemoglobin and cytochrome C derived from equine heart frozen down to 77.15 K with 1–2 deg/min and 300 deg/min rate with following thawing on a water bath at 293.15 K. Native proteins were assumed as a control. Influence of freeze-thawing protocols on protein structure was investigated using spectrophotometric and fluorescent assays. Antioxidant activities of isolated proteins were estimated by their ability to reduce ABTS ⁺ radical. It has been established that unfolding derived from freeze-thawing exposure leads to protein antioxidant activity increasing while decreasing of such an activity may be connected with macromolecule aggregation. Character of freeze-thawing influence on antioxidant activity of proteins depends on molecule structure peculiarities and freezing protocols.
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| Date |
2018-01-19T20:23:51Z
2018-01-19T20:23:51Z 2017 |
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| Type |
Article
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| Identifier |
Influence of freezing down to 77.15 K on structure and antioxidant power of some proteins / S.L. Rozanova, S.V. Narozhnyi, O.A. Nardid // Физика низких температур. — 2017. — Т. 43, № 6. — С. 898-901. — Бібліогр.: 18 назв. — англ.
0132-6414 PACS: 33.20.–t, 33.20.Lg, 33.50.Dq http://dspace.nbuv.gov.ua/handle/123456789/129506 |
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| Language |
en
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| Relation |
Физика низких температур
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| Publisher |
Фізико-технічний інститут низьких температур ім. Б.І. Вєркіна НАН України
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