Influence of two pt(iv) complexes on viability, apoptosis and cell cycle of B16 mouse melanoma tumors
Vernadsky National Library of Ukraine
Переглянути архів Інформація| Поле | Співвідношення | |
| Title |
Influence of two pt(iv) complexes on viability, apoptosis and cell cycle of B16 mouse melanoma tumors
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| Creator |
Kaznica, A.
Drewa, T. Lakomska, I. Ryta-Stamirowska, P. Debski, R. Styczynski, J. Drewa, G. Szłyk, E. |
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Original contributions
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| Description |
Several platinum(IV) complexes are showing considerable promise in initial trials, producing reactive intermediates that then interact with DNA. Aim: To perform in vitro study of two new platinum(IV) complexes cytotoxic effect on B16 mouse melanoma cells. Methods: PtCl₄ (dbtp)₂ and PtCl₂ (6mp)₂ complexes were prepared. PtCl₄ (dbtp)₂ was created as modification of PtCl₄ (dmtp) test previously.Apoptosis and necrosis were examined using flow cytometry, upon Annexin V/PI staining. Results: LC₁₀,LC₅₀ andLC₉₀ parameters established for PtCl₄ (dbtp)₂ were as following: 2.6, 17.0, 58.0 μmol/L. However LC₁₀ andLC₅₀ established for PtCl₂ (6mp)₂ were 1.2 and 14.0μmol/l respectively. The both complexes induced apoptosis. PtCl₂ (6mp)₂ induced cell cycle arrest in G0/G1, while PtCl₄ (dbtp)₂ — in S-phase. Conclusions: PtCl₄ (dbtp)₂ appeared to be more cytotoxic against B16 cells than PtCl₂ (6mp)₂ . Apoptosis was the main mechanism of cell loss in cultures incubated with both tested complexes. |
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| Date |
2018-06-14T15:40:56Z
2018-06-14T15:40:56Z 2009 |
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| Type |
Article
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| Identifier |
Influence of two pt(iv) complexes on viability, apoptosis and cell cycle of B16 mouse melanoma tumors / A. Kaznica, T. Drewa, I. Lakomska, P. Ryta-Stamirowska, R. Debski, J. Styczynski, G. Drewa, E. Szłyk // Experimental Oncology. — 2009. — Т. 31, № 1. — С. 33–36. — Бібліогр.: 19 назв. — англ.
1812-9269 http://dspace.nbuv.gov.ua/handle/123456789/135114 |
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| Language |
en
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| Relation |
Experimental Oncology
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| Publisher |
Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України
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