Expression profile of nuclear receptors upon epstein — barr virus induced b cell transformation
Vernadsky National Library of Ukraine
Переглянути архів Інформація| Поле | Співвідношення | |
| Title |
Expression profile of nuclear receptors upon epstein — barr virus induced b cell transformation
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| Creator |
Yenamandra, S.P.
Lundin, A. Arulampalam, V. Yurchenko, M. Pettersson, S. Klein, G. Kashuba, E. |
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| Subject |
Original contributions
Original contributions |
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| Description |
Background: Infection of human B cells with Epstein—Barr virus (EBV) induces metabolic activation, morphological transformation, cell proliferation and eventual immortalization. Aim: To identify the nuclear receptors, which are the cellular interaction partners of EBNAs, that will help to elucidate the mechanism of B cell transformation. Methods: We have compared the nuclear receptor profile in the naïve and EBV-transformed B-lymphocytes, using TaqMan LDA microfluidic card technology. Results: Out of 48 nuclear receptor, 17 showed differential expression at the mRNA level. The expression of 5 genes was elevated in EBV-transformed cells, whereas 12 genes were downregulated in lymphoblastoid cells (LCLs). 7 genes were studied at the protein level; 2 genes were up regulated (Nr2F2 and RARA) and 4 genes were down regulated (ERB, NUR77, PPARG, and VDR) in LCLs. Conclusion: The nuclear receptor profiling on EBV infected B cells showed alterations of nuclear receptors expression at both mRNA and protein levels compared with non infected peripheral blood cells. Further analysis on a possible role of each nuclear receptor in EBV induced cell transformation should be performed. |
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| Date |
2018-06-15T14:02:50Z
2018-06-15T14:02:50Z 2009 |
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| Type |
Article
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| Identifier |
Expression profile of nuclear receptors upon epstein — barr virus induced b cell transformation / S.P. Yenamandra, A. Lundin1, V. Arulampalam, M. Yurchenko, S. Pettersson, G. Klein, E. Kashuba // Experimental Oncology. — 2009. — Т. 31, № 2. — С. 92–96. — Бібліогр.: 23 назв. — англ.
1812-9269 http://dspace.nbuv.gov.ua/handle/123456789/135700 |
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| Language |
en
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| Relation |
Experimental Oncology
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| Publisher |
Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України
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