Modifying effects of 5-azacytidine on metal-containing proteins profile in guerin carcinoma with different sensitivity to cytostatics
Vernadsky National Library of Ukraine
Переглянути архів Інформація| Поле | Співвідношення | |
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Modifying effects of 5-azacytidine on metal-containing proteins profile in guerin carcinoma with different sensitivity to cytostatics
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| Creator |
Chekhun, V.F.
Lozovska, Y.V. Naleskina, L.A. Borikun, T.V. Burlaka, A.P. Todor, I.N. Demash, D.V. Yalovenko, T.M. Zadvornyi, T.V. Pavlova, A.O. Storchay, D.M. Lukianova, N.Yu. |
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Original contributions
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| Description |
Aim: To assess the influence of the treatment with 5-azacytidine (5-aza) on the profile of metal-containing proteins and factors of their regulation in Guerin carcinoma cells in vivo. Materials and Methods: The study was conducted on Wistar rats transplanted with wild-type Guerin carcinoma (Guerin/WT) and its strains resistant to cisplatin (Guerin/CP) or doxorubicin (Guerin/Dox). Animals were distributed in 6 groups treated with 5-aza and control animals without treatment. 5-Aza was injected by i.v. route (1 injection in 4 days at a dose of 2 mg/kg starting from the 4th day after tumor transplantation, 4 injections in total). Ferritin levels in blood serum and tumor tissue were measured by ELISA, transferrin and free iron complexes — by low-temperature EPR, miRNA-200b, -133a and -320a levels and promoter methylation — by real-time quantitative reverse transcription polymerase chain reaction. Results: The study has shown that 5-aza treatment caused demethylation of promoter regions of fth1 and tfr1 genes in all studied Guerin carcinoma strains. 5-Aza treatment resulted in a significant decrease of ferritin levels in tumor tissue (by 32.1% in Guerin/WT strain, by 29.8% in Guerin/Dox and by 69.1% in Guerin/CP). These events were accompanied by 3.5-fold and 2-fold increase of free iron complexes levels in tumor tissue of doxorubicin and cisplatin resistant strains, respectively. Also, 5-aza treatment resulted in significantly elevated levels of miR-200b, -133a, 320a expression in tumor tissue. After 5-aza treatment, ferritin levels in blood serum of animals with Guerin/Dox were increased by 23.9%, while in Guerin/Wt and Guerin/CP they were decreased by 17 and 16%, respectively. Conclusion: Alterations of epigenetic regulation upon in vivo treatment with 5-aza change the levels of metal-containing proteins due to DNA demethylation and altered miRNA expression profiles in Guerin carcinoma cells.
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| Date |
2018-06-17T15:11:58Z
2018-06-17T15:11:58Z 2016 |
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Article
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| Identifier |
Modifying effects of 5-azacytidine on metal-containing proteins profile in guerin carcinoma with different sensitivity to cytostatics / V.F. Chekhun, Y.V. Lozovska, L.A. Naleskina, T.V. Borikun, A.P. Burlaka, I.N. Todor, D.V. Demash, T.M. Yalovenko, T.V. Zadvornyi, A.O. Pavlova, D.M. Storchay, N.Yu. Lukianova // Experimental Oncology. — 2016 — Т. 38, № 4. — С. 283-287. — Бібліогр.: 16 назв. — англ.
1812-9269 http://dspace.nbuv.gov.ua/handle/123456789/137697 |
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en
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| Relation |
Experimental Oncology
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| Publisher |
Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України
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