Parthenolide reduces gene transcription of prosurvival mediators in U937 cells
Vernadsky National Library of Ukraine
Переглянути архів Інформація| Поле | Співвідношення | |
| Title |
Parthenolide reduces gene transcription of prosurvival mediators in U937 cells
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| Creator |
Mohammadi, S.
Zahedpanah, M. Nikbakht, M. Shaiegan, M. Hamidollah Ghaffari, S. Nikugoftar, M. Rahmani, B. Hamedi Asl, D. |
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Original contributions
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| Description |
In acute myeloid leukemia (AML) the functional abnormalities of osteopontin (OPN), NF-kB, PI3K/AKT/mTOR/PTEN pathway or β-catenin have been considered. Aim: To analyze the response of U937 cells to parthenolide (PTL) through the involvement of expression of OPN protein, RelB, AKT1, mTOR, PTEN and β-catenin genes. Materials and Methods: The U937 cells were treated with PTL at concentrations of 4 μM (IC25) or 6 μM (IC50) and with OPN siRNA for MTT assay and colony forming assay. Western blot analysis using antibodies against OPN was performed with lysates of PTL-treated cells. Quantitative real-time polymerase chain reaction was performed using primers for OPN siRNA, RelB, AKT1, mTOR, PTEN and β-catenin. Results: PTL reduces OPN protein level and down-regulates RelB mRNA in U937 cell line. Suppression of OPN with siRNA increases the cytotoxic effects of PTL. Also, mRNA expression of AKT1, mTOR, PTEN, and β-catenin decreases with PTL or OPN siRNA. Conclusion: Sensitivity of U937 cells to PTL can be associated with the reduction in expression of prosurvival mediators.
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| Date |
2018-06-17T14:01:40Z
2018-06-17T14:01:40Z 2017 |
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| Type |
Article
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| Identifier |
Parthenolide reduces gene transcription of prosurvival mediators in U937 cells / S. Mohammadi, M. Zahedpanah, M. Nikbakht, M. Shaiegan, S. Hamidollah Ghaffari, M. Nikugoftar, B. Rahmani, D. Hamedi Asl // Experimental Oncology. — 2017 — Т. 39, № 1. — С. 30-35. — Бібліогр.: 55 назв. — англ.
1812-9269 http://dspace.nbuv.gov.ua/handle/123456789/137611 |
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| Language |
en
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| Relation |
Experimental Oncology
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| Publisher |
Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України
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