Anti-histone H1 IgGs possess proliferative activity towards human T-leukaemia cem cells
Vernadsky National Library of Ukraine
Переглянути архів Інформація| Поле | Співвідношення | |
| Title |
Anti-histone H1 IgGs possess proliferative activity towards human T-leukaemia cem cells
|
|
| Creator |
Kit, Yu.
Magorivska, I. Bilyi, R. Myronovskij, S. Stoika, R. |
|
| Subject |
Original contributions
|
|
| Description |
The aim of this study was to characterize the proliferative activity of the anti-histone H1 IgGs towards human T-leukaemia CEM cells. Materials and Methods: Anti-histone H1 IgGs were purified from blood serum of systemic lupus erythematosus patients by precipitation of serum proteins with 50% ammonium sulfate followed by a sequential affinity chromatography on Protein GSepharose and histone H1-Sepharose columns. To avoid contamination with other proteins, anti-histone H1 IgGs were subjected to strongly acidic pH 2.0 during gel filtration through HPLC column. The effects of the anti-histone H1 IgGs on cell viability and cell cycle were tested by MTS-assay and flow cytometry, correspondingly. The cross-reactivity of the anti-histone H1 antibodies towards heterogenetic and cellular antigens was evaluated by Western-blot analysis. Results: It was found that incubation of CEM cells with the HPLC-purified anti-histone H1 IgGs resulted in significant stimulation of cell growth by 46% after 48 h of incubation. These IgGs possess an antigenic poly-specificity to positively charged heterogenetic antigens and different cellular antigens. FITC-labeled and biotinylated anti-histone H1 IgGs are internalized by CEM cells and preferentially accumulated in the cytoplasm. Conclusion: The anti-histone H1 IgGs are shown to internalize human T-leukemia CEM and stimulate their proliferation. These IgGs are polyspecific toward cellular antigens.
|
|
| Date |
2018-06-17T13:54:51Z
2018-06-17T13:54:51Z 2017 |
|
| Type |
Article
|
|
| Identifier |
Anti-histone H1 IgGs possess proliferative activity towards human T-leukaemia cem cells / Yu. Kit1, I. Magorivska, R. Bilyi, S. Myronovskij, R. Stoika // Experimental Oncology. — 2017 — Т. 39, № 1. — С. 36-41. — Бібліогр.: 34 назв. — англ.
1812-9269 http://dspace.nbuv.gov.ua/handle/123456789/137602 |
|
| Language |
en
|
|
| Relation |
Experimental Oncology
|
|
| Publisher |
Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України
|
|