Antiangiogenic properties of a nutrient mixture in a model of hemangioma
Vernadsky National Library of Ukraine
Переглянути архів Інформація| Поле | Співвідношення | |
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Antiangiogenic properties of a nutrient mixture in a model of hemangioma
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| Creator |
Roomi, M.W.
Kalinovsky, T. Niedzwiecki, A. Rath, M. |
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| Subject |
Original contributions
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| Description |
The pathogenesis of hemangiomas is still largely unknown and the current therapy, such as systemic corticosteroid, vincristine, and interferon-alpha, is toxic and remains unsatisfactory. A nutrient mixture (NM) containing lysine, proline, ascorbic acid and green tea extract has shown significant anti-angiogenic and anti-tumor effect against a number of cancer cell lines. Aim: Using a mouse hemangioendothelioma model, we investigated the efficacy of NM. We also tested the effect of NM in vitro, evaluating cell viability, MMP secretion, invasion, morphology and apoptosis. Methods: Athymic nude mice, 5–6 weeks old, were inoculated with 3 x106 EOMA cells subcutaneously and randomly divided into two groups; group A was fed a regular diet and group B — a regular diet supplemented with 0.5% NM. Four weeks later, the mice were sacrificed and their tumors were excised, weighed and processed for histology. We also tested the effect of NM in vitro. Results: NM inhibited the growth of tumors by 50%. In vitro, NM exhibited dose response cytotoxicity with 10%, 30% and 55% at 10, 100 and 1000 μg/ml. Invasion through Matrigel was inhibited at 50, 100 and 500 μg/ml by 25%, 30% and 100% respectively. NM induced dose-dependent apoptosis of EOMA cells. Conclusions: These results suggest that NM may have therapeutic potential in treating infantile hemangioendotheliomas and, perhaps, other cutaneous vascular tumors.
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| Date |
2018-06-18T10:59:38Z
2018-06-18T10:59:38Z 2009 |
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| Type |
Article
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| Identifier |
Antiangiogenic properties of a nutrient mixture in a model of hemangioma / M.W. Roomi, T. Kalinovsky, A. Niedzwiecki, M. Rath // Experimental Oncology. — 2009. — Т. 31, № 4. — С. 214-219. — Бібліогр.: 33 назв. — англ.
1812-9269 http://dspace.nbuv.gov.ua/handle/123456789/138207 |
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| Language |
en
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| Relation |
Experimental Oncology
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Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України
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