Immunocytochemical study of BCR and bcr-abl localization in K562 cells
Vernadsky National Library of Ukraine
Переглянути архів Інформація| Поле | Співвідношення | |
| Title |
Immunocytochemical study of BCR and bcr-abl localization in K562 cells
|
|
| Creator |
Telegeev, G.D.
Dubrovska, A.N. Nadgorna, V.A. Dybkov, M.V. Zavelevich, M.P. Maliuta, S.S. Gluzman, D.F. |
|
| Subject |
Original contributions
|
|
| Description |
Aim: To obtain polyclonal antibodies against recombinant proteins recognizing Bcr domain and fusion region of Bcr-Abl and analyze the patterns of intracellular distribution of Bcr and Bcr-Abl proteins in K562 cells of chronic myelogenous leukemia. Methods: The coding sequences of DН and РН domains of Bcr-Abl were cloned, and the recombinant proteins were expressed in E. coli. The rabbit polyclonal antibodies were produced and used for immunocytochemical study of Bcr and Bcr-Abl localization in K562 cells. Results: The gene constructs containing sequences coding for DН and РН domains of Bcr-Abl have been obtained. The antibodies with relative specificity to corresponding recombinant proteins differ by the patterns of their intracellular reactivity with Bcr- and Bcr-Abl related structures. While Bcr protein is located predominantly perinuclearly, antibody against hybrid Bcr-Abl protein is reacted with the structures in cell periphery, namely on cell membranes. Conclusion: Antibodies against DН and РН domains of Bcr-Abl react with proteins located differently in chronic myelogenous leukemia cells. The difference in intracellular localization of Bcr and Bcr-Abl may be attributable to the different domains interacting with different multiprotein complexes.
|
|
| Date |
2018-06-19T10:23:34Z
2018-06-19T10:23:34Z 2010 |
|
| Type |
Article
|
|
| Identifier |
Immunocytochemical study of BCR and bcr-abl localization in K562 cells / G.D. Telegeev, A.N. Dubrovska, V.A. Nadgorna, M.V. Dybkov, M.P. Zavelevich, S.S. Maliuta, D.F. Gluzman // Experimental Oncology. — 2010. — Т. 32, № 2. — С. 81-83. — Бібліогр.: 13 назв. — англ.
1812-9269 http://dspace.nbuv.gov.ua/handle/123456789/138603 |
|
| Language |
en
|
|
| Relation |
Experimental Oncology
|
|
| Publisher |
Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України
|
|