Kinase suppressor of Ras 2 is involved in regulation of cell proliferation and is up-regulated in human invasive ductal carcinomas of breast
Vernadsky National Library of Ukraine
Переглянути архів Інформація| Поле | Співвідношення | |
| Title |
Kinase suppressor of Ras 2 is involved in regulation of cell proliferation and is up-regulated in human invasive ductal carcinomas of breast
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| Creator |
Jia, M.
Souchelnytskyi, S. |
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Original contributions
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| Description |
Aim: To study the expression of Kinase Suppressor of Ras 2 (KSR2) in human breast tumors and its effect on proliferation of breast epithelial cells. We reported previously that KSR2 was up-regulated in immortalized human breast epithelial cells. Methods: Proteomics technologies, systems biology tool for a KSR2 network analysis, immunoblotting, siRNA technology, overexpression of KSR2, cell proliferation assays and immunohistochemistry of tissue microarray of human breast tumors and normal breast tissue were used. Results: In conditionally immortalized primary epithelial cells KSR2 expression was shown to be up-regulated. The involvement of KSR2 in regulation of cell proliferation was predicted by a KSR2-centered network analysis. We observed that KSR2 down-regulation with specific siRNA inhibited cell proliferation. By immunohistochemistry of tissue microarray it was demon strated that KSR2 expression was enhanced in human invasive breast carcinomas. Conclusion: Our findings propose KSR2 as a new marker of immortalization, which has an impact on cell proliferation.
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| Date |
2018-06-19T10:30:30Z
2018-06-19T10:30:30Z 2010 |
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| Type |
Article
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| Identifier |
Kinase suppressor of Ras 2 is involved in regulation of cell proliferation and is up-regulated in human invasive ductal carcinomas of breast / M. Jia, S. Souchelnytskyi // Experimental Oncology. — 2010. — Т. 32, № 3. — С. 209-212. — Бібліогр.: 12 назв. — англ.
1812-9269 http://dspace.nbuv.gov.ua/handle/123456789/138608 |
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en
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| Relation |
Experimental Oncology
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| Publisher |
Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України
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