Запис Детальніше

TGF-β1 expression by glioma C6 cells in vitro

Vernadsky National Library of Ukraine

Переглянути архів Інформація
 
 
Поле Співвідношення
 
Title TGF-β1 expression by glioma C6 cells in vitro
 
Creator Liubich, L.D.
Kovalevska, L.M.
Lisyany, M.I.
Semenova, V.M.
Malysheva, T.A.
Stayno, L.P.
Vaslovych, V.V.
 
Subject Original contributions
 
Description The aim of the work was to study the impact of fetal rat brain cell supernatant (FRBCS) on the expression of transforming growth factor β1 (TGF-β1) and p53 in C6 cells of rat glioma in vitro. Materials and Methods: FRBCS was obtained from suspensions of fetal rat brain cells on the 14th (E14) day of gestation. C6 glioma cells were cultured for 48 h in the presence of FRBCS or FRBCS + anti-TGF-β1 monoclonal antibody. Immunocytochemical staining for TGF-β1 and p53 was performed. Results: The proportion of TGF-β1-immunopositive tumor cells in C6 glioma cultures was statistically significantly higher than in the control cell cultures of normal fetal rat brain. FRBCS reduced the proportion of TGF-β1-immunopositive tumor cells and increased the proportion of p53-immunopositive cells in C6 glioma cultures. In cells cultured with FRBCS + anti-TGF-β1 monoclonal antibody, the above effects of FRBCS were abrogated. Conclusion: The obtained results suggest that TGF-β1 seems to be responsible for decrease in TGF-β1 expression and increase in p53 expression in C6 glioma cells treated with FRBCS.
 
Date 2018-06-19T10:00:39Z
2018-06-19T10:00:39Z
2017
 
Type Article
 
Identifier TGF-β1 expression by glioma C6 cells in vitro / L.D. Liubich, L.M. Kovalevska, M.I. Lisyany, V.M. Semenova, T.A. Malysheva, L.P. Stayno, V.V. Vaslovych // Experimental Oncology. — 2017 — Т. 39, № 4. — С. 258–263. — Бібліогр.: 40 назв. — англ.
1812-9269
http://dspace.nbuv.gov.ua/handle/123456789/138583
 
Language en
 
Relation Experimental Oncology
 
Publisher Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України