Anti-cancer efficiency of natural killer cells differentiated from human adipose tissue-derived mesenchymal stem cells and transfected with miRNA150
Vernadsky National Library of Ukraine
Переглянути архів Інформація| Поле | Співвідношення | |
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Anti-cancer efficiency of natural killer cells differentiated from human adipose tissue-derived mesenchymal stem cells and transfected with miRNA150
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| Creator |
Karlitepe, A.
Kabadayi, H. Vatansever, S. Gurdal, M. Gunduz, C. Ercan, G. |
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Original contributions
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| Description |
Aim: The aim of this study is to investigate the effects of miR150 transfection on NK-like cells differentiated from adipose tissue derived mesenchymal stem cells (AD-MSCs). Methods: NK-like cells were differentiated from AD-MSCs and activated by miR150 transfection. Transfected/non-transfected NK-like cells were characterized by immunohistochemical and RTPCR analyzes. Apoptotic efficiency of the transfected/non-transfected NK-like cells on pancreatic cancer cells PANC1 were determined by TUNEL and RT-PCR. Results: In miR150-transfected cells, the increased expression of NK cell-specific genes such as GZMB, KIR2DL2, CD16, CD56, NKG2D, NKp46 and increased immunoreactivity of NK cell-specific surface marker CD314 (NKG2D) were evident. TUNEL assays showed that NK-like cells with/without transfection induced apoptosis in PANC1 cells in the same manner. The decrease in oncogene expression and the increase in the tumor suppressor gene expression in PANC1 cells upon co-culture with NK-like cells differentiated from AD-MSCs were more prominent following miRNA150 transfection. Conclusion: It was shown in vitro that NK-like cells could be obtained by differentiation from AD-MSCs and their efficiency could be increased via miR150 transfection. The results are encouraging for further clinical studies in improvement of immunotherapeutic approaches for cancer therapy.
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| Date |
2018-06-19T09:11:27Z
2018-06-19T09:11:27Z 2017 |
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| Type |
Article
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| Identifier |
Anti-cancer efficiency of natural killer cells differentiated from human adipose tissue-derived mesenchymal stem cells and transfected with miRNA150 / A. Karlitepe, H. Kabadayi, S. Vatansever, M. Gurdal, C. Gunduz, G. Ercan // Experimental Oncology. — 2017 — Т. 39, № 3. — С. 212–218. — Бібліогр.: 32 назв. — англ.
1812-9269 http://dspace.nbuv.gov.ua/handle/123456789/138541 |
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en
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Experimental Oncology
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| Publisher |
Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України
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