Isolectins of phytohemagglutinin are able to induce apoptosis in HEp-2 carcinoma cells in vitro
Vernadsky National Library of Ukraine
Переглянути архів Інформація| Поле | Співвідношення | |
| Title |
Isolectins of phytohemagglutinin are able to induce apoptosis in HEp-2 carcinoma cells in vitro
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| Creator |
Kochubei, T.O.
Maksymchuk, O.V. Piven, O.O. Lukash, L.L. |
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| Subject |
Original contributions
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| Description |
Aim: To study the effects of total phytohemagglutinin (PHA) and its isolectins on cell death and apoptosis in human HEp-2 carcinoma cells and to analyze the possible molecular mechanisms of lectin induced apoptosis. Materials and Methods: The commercial preparation of the kidney beans (Phaseolus vulgaris) lectins and HEp-2 cells were used. Apoptosis index was determined using acridine orange and ethidium bromide staining. The expression levels of apoptosis mediator cleaved caspase-3 and proapoptotic Bax protein were studied by Western blot analysis. The gene expression levels were analyzed by qPCR. Results: PHA and its isolectins induced apoptosis in HEp-2 cells accompanied by the increased expression of caspase-3 cleaved form, with PHA-E being the most effective. The treatment of HEp-2 cells with PHA or its isolectins resulted in a marked increase of Bax on both mRNA and protein levels. Conclusions: PHA and its isolectins were shown to induce the apoptosis in human HEp-2 carcinoma cells via increasing proapoptotic protein Bax and activating caspases-3. Key Words: phytohemagglutinin, isolectins, apoptosis, caspase-3, Bax.
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| Date |
2019-01-22T11:32:48Z
2019-01-22T11:32:48Z 2015 |
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| Type |
Article
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| Identifier |
Isolectins of phytohemagglutinin are able to induce apoptosis in HEp-2 carcinoma cells in vitro / T.O. Kochubei, O.V. Maksymchuk, O.O. Piven, L.L. Lukash // Experimental Oncology. — 2015. — Т. 37, № 2. — С. 116-119. — Бібліогр.: 15 назв. — англ.
1812-9269 http://dspace.nbuv.gov.ua/handle/123456789/145466 |
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| Language |
en
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| Relation |
Experimental Oncology
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| Publisher |
Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України
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