Antitumor and genotoxic effects of lactoferrin in Walker-256 tumor-bearing rats
Vernadsky National Library of Ukraine
Переглянути архів Інформація| Поле | Співвідношення | |
| Title |
Antitumor and genotoxic effects of lactoferrin in Walker-256 tumor-bearing rats
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| Creator |
Chekhun, V.F.
Storchai, D.M. Todor, I.N. Borikun, T.V. Lukianova, N.Yu. |
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Original contributions
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| Description |
Aim: To investigate the influence of exogenous lactoferrin (LF) on tumor growth, energy and lipid metabolism of Walker-256 carcinosarcoma and to assess genotoxic effects of LF. Materials and Methods: The study was performed on Walker-256 tumor-bearing rats. Total lipids and phospholipids were determined by thin-layer chromatography. Comet assay was used to investigate the genotoxic effects of LF. Results: Daily i.p. administrations of exogenous LF at concentrations of 1 mg/kg and 10 mg/kg starting from the 4th day after tumor transplantation suppressed growth of Walker-256 carcinosarcoma by almost 44%. After treatment with recombinant LF in both doses, the phospholipid composition of Walker-256 carcinosarcoma cells was changed (3-fold increase of phosphatidylethanolamine, 3.4-fold increase of phosphatidylcholine, and 1.8-fold increase of sphingomyelin, while the cardiolipin content decreased by 67%. Exogenous LF was not genotoxic for bone marrow cells (as assessed by the ratio of PCE/NCE, number of micronuclei) and peripheral blood lymphocytes (percentage of DNA in the tail of a comet) in Walker-256 carcinosarcoma-bearing rats. Conclusion: Exogenous LF caused the inhibition of Walker-256 carcinosarcoma growth and a decrease in the microviscosity of plasma cell membranes, and exerted no genotoxicity toward bone marrow cells and peripheral blood of experimental animals. Key Words: lactoferrin, breast cancer, Walker-256 carcinosarcoma, phospholipid content, genotoxicity.
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| Date |
2019-01-26T21:05:52Z
2019-01-26T21:05:52Z 2018 |
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| Type |
Article
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| Identifier |
Antitumor and genotoxic effects of lactoferrin in Walker-256 tumor-bearing rats / V.F. Chekhun, D.M. Storchai, I.N. Todor, T.V. Borikun, N.Yu. Lukianova // Experimental Oncology. — 2018 — Т. 40, № 3. — С. 200–204. — Бібліогр.: 28 назв. — англ.
1812-9269 http://dspace.nbuv.gov.ua/handle/123456789/145710 |
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| Language |
en
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| Relation |
Experimental Oncology
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| Publisher |
Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України
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