Stainless steel electrode for sensitive luminol electrochemiluminesce
Електронного архіву Харківського національного університету радіоелектроніки (Open Access Repository of KHNURE)
Переглянути архів Інформація| Поле | Співвідношення | |
| Title |
Stainless steel electrode for sensitive luminol electrochemiluminesce
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| Creator |
Zholudov, Yu.
Shimeles Addisu Kitte Wenyue Gao Anaclet Nsabimana Zhongyuan Liu Guobao Xu Liming Qi |
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| Subject |
chemiluminescence electrogenerated, stainless steel electrode, H2O2 detection
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| Description |
The electrogenerated chemiluminescence (ECL) application of stainless steel, a robust and cost-effective material, has been developed for the first time. The type 304 stainless steel electrode shows appealing ECL performance for luminol-H2O2 system. It enables the detection of H2O2 with a linear range from 1 to 1000 nM and a limit of detection of0.456 nM (S/N = 3). The ECL method based on type 304 stainless steel electrode is more sensitive, more cost-effective,and much simpler than other ECL methods reported before. Because the stainless steel electrode has excellent performance for H2O2 detection and H2O2 participates in many important enzymatic reactions, the applications of stainless steel electrode-based ECL for the detection of enzyme activities and enzyme substrates were further investigated using glucoseoxidase (GODx) and glucose as representative enzyme and substrate. The concentrations of glucose and the activity of GODx were directly proportional to the ECL intensities over a range of 0.1 – 1000 µM and 0.001 – 0.7 U/mL with a limit of detections of 0.076 μM and 0.00087 U/mL (S/N = 3), respectively. This method was successfully used for determining glucose in honey. Because of its remarkable performance and user-friendly features, stainless steel electrode holds great promise in various electroanalytical applications, such as biosensing, disposable sensors, and wearable sensors. |
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| Date |
2017-11-03T07:13:27Z
2017-11-03T07:13:27Z 2017 |
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| Type |
Article
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| Identifier |
Stainless steel electrode for sensitive luminol electrochemiluminescence detection of H2O2, glucose, and glucose oxidase activity Shimeles Addisu Kitte, Wenyue Gao, Yuriy T. Zholudov, Liming Qi, Anaclet Nsabimana, Zhongyuan Liu, and Guobao Xu Anal. Chem., Just Accepted Manuscript • DOI: 10.1021/acs.analchem.7b01939 • Publication Date (Web): 23 Aug 2017
http://openarchive.nure.ua/handle/document/4067 |
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| Language |
en
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| Publisher |
Subscriber access provided by UNIVERSITY OF ADELAIDE LIBRARIES
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