Conductometric urease microbiosensor based on thin-film interdigitated electrodes for urea determination
Vernadsky National Library of Ukraine
Переглянути архів ІнформаціяПоле | Співвідношення | |
Title |
Conductometric urease microbiosensor based on thin-film interdigitated electrodes for urea determination
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Creator |
Dzyadevich, S.V.
Zhylyak, G.A. Soldatkin, A.P. |
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Description |
The characteristics of conductometric urease microbiosensor based on thin-film interdigitated electrodes for urea determination have been studied. Urease has been immobilized by cross-linking with bovine serum albumin using glutaraldehyde. The resulting conductivity changes are produced by enzymatically catalyzed hydrolysis of urea. This process for both immobilized enzyme and soluble one is described by the classic laws of enzymatic kinetics. The influence of ionic strength, pH and buffer capacity of the samples on the biosensor response has been thoroughly tested. The results have been discussed concerning urea concentration analysis in human blood.
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Date |
2019-06-14T12:12:07Z
2019-06-14T12:12:07Z 1996 |
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Type |
Article
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Identifier |
Conductometric urease microbiosensor based on thin-film interdigitated electrodes for urea determination / S.V. Dzyadevich, G.A. Zhylyak, A.P. Soldatkin // Биополимеры и клетка. — 1996. — Т. 12, № 1. — С. 53-57. — Бібліогр.: 9 назв. — англ.
0233-7657 http://dspace.nbuv.gov.ua/handle/123456789/153663 577.15+573.6 |
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Language |
en
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Relation |
Биополимеры и клетка
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Publisher |
Інститут молекулярної біології і генетики НАН України
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